CORONAVIRUS-LIKE PARTICLES IN DIARRHOEA STOOLS

Ribonucleoprotein-like structures from coronavirus particles.

The structure of the ribonucleoprotein (RNP) complex of three coronaviruses was investigated. A single-stranded helix of diam. 14 to 16 nm and up to 320 nm in length was released from disrupted particles of human coronavirus strain 229E and mouse hepatitis virus strain 3 after incubation in mild conditions. The helical complexes appeared to be composed of globular subunits with long axes of 5 t...

Culture negative cytotoxin positive stools in community acquired diarrhoea.

In a significant proportion of cases of community acquired diarrhoea no recognised pathogen is detected. Verocytotoxin producing Escherichia coli (VTEC) are associated with haemorrhagic colitis and the haemolytic uraemic syndrome but their role in community acquired diarrhoea is not fully understood. Using a method of toxin enhancement in mixed faecal culture, 175 stools negative on culture for...

Avian lymphoid leukosis: Virus-like particles in kidney Cells

Intestinal permeability changes and excretion of micro-organisms in stools of infants with diarrhoea and vomiting.

The relation between diarrhoea and vomiting, the excretion of stool micro-organisms, and the passive intestinal permeability in 20 infants living in a deprived urban area was studied prospectively from birth to age 6 months. Intestinal permeability was measured from the ratio of lactulose to mannitol recovered in the urine of infants receiving feeds containing both markers. Micro-organism excre...

Cytokines in stools of children with inflammatory bowel disease or infective diarrhoea.

AIMS To determine the concentrations of interleukin-6 (IL-6) and tumour necrosis factor alpha (TNF alpha) in stools from children. METHODS Stool samples from 14 healthy children, 32 children with inflammatory bowel disease, and 23 children with acute diarrhoea were emulsified in an equal volume of phosphate buffered saline and then centrifuged to produce a clear supernatant fluid. IL-6 and TN...